Washing scrubs at home does not ensure sufficient bacteria decontamination. (Pixel-Shot/Shutterstock)
In a nutshell
- Many home washing machines don’t get hot enough to kill dangerous bacteria. Half of the machines tested failed to reach or maintain the 60°C (140°F) needed for effective disinfection, especially during quick wash cycles.
- Washing machines can harbor antibiotic-resistant bacteria. Researchers found potentially harmful microbes like Pseudomonas, Acinetobacter, and Mycobacterium inside household washers, along with genes that help bacteria resist antibiotics.
- Detergents may be making things worse. When bacteria were repeatedly exposed to common laundry detergents, they developed resistance not only to the detergent but also to critical antibiotics, including carbapenems and those used to treat MRSA.
LEICESTER, England — Healthcare workers’ uniforms washed at home might be bringing dangerous bacteria back to hospitals, creating a breeding ground for drug-resistant superbugs. A new study from researchers in England reveals that most domestic washing machines fail to properly disinfect clothes, potentially endangering patients and contributing to the growing crisis of antibiotic resistance.
The study, published in PLOS One, revealed that more than half of household washing machines don’t reach high enough temperatures to kill dangerous bacteria, even when set to high temperature cycles. What’s worse, the bacteria surviving these washes can develop resistance to common antibiotics, creating potential superbugs that could make their way back into healthcare settings.
An Overlooked Infection Risk
Researchers at De Montfort University tested both the bacteria-killing ability of home washers and the microbes lurking inside them. While hospital linen and scrubs undergo carefully controlled industrial washing processes to eliminate microbes, healthcare worker uniforms often get laundered at home under much less rigorous conditions.
This is a blind spot in infection control efforts. While hospitals focus heavily on hand hygiene and surface disinfection, the textiles worn by healthcare workers receive less attention, despite evidence showing bacteria can survive for weeks on fabric.
Dr. Caroline Cayrou, CC-BY 4.0 https://creativecommons.org/licenses/by/4.0/)
The research team tested six different household washing machines with a biological indicator containing Enterococcus faecium, a common hospital-acquired infection. They ran both full-length and quick wash cycles at 60°C (140°F), the temperature recommended by UK health guidelines.
Only half of the machines achieved sufficient decontamination during full-length washes, and performance was even worse during quick cycles. One machine never exceeded 20°C (68°F) despite being set to a 60°C cycle.
The researchers found that only two machines maintained their peak temperature above 57°C (134.6°F) for the recommended 10 minutes, though even these fell short of the recommended 60°C threshold.
The Hidden Ecosystem Inside Your Washing Machine
But temperature issues aren’t the only problem. The team analyzed the microbial populations inside 12 different home washing machines and found they harbor potentially dangerous bacteria, including species of Mycobacterium, Pseudomonas, and Acinetobacter—all potential pathogens.
These bacteria also carried genes for antibiotic resistance. The researchers detected genes encoding for efflux pumps (which help bacteria push antibiotics out of their cells) and mechanisms that alter antibiotic targets, making the drugs ineffective.
To test whether the detergents themselves might contribute to antibiotic resistance, the team exposed common hospital pathogens—Staphylococcus aureus, Klebsiella pneumoniae, and Pseudomonas aeruginosa—to increasing concentrations of laundry detergents.
The bacteria quickly adapted to survive higher and higher concentrations. S. aureus, for example, developed tolerance to liquid detergent concentrations almost 90,000% higher than what initially inhibited its growth. K. pneumoniae‘s tolerance increased by over 1.5 million percent.
Bacteria exposed to detergents developed cross-resistance to critical medical antibiotics. K. pneumoniae became resistant to carbapenems, often considered “last-resort” antibiotics. S. aureus developed resistance patterns resembling those of MRSA (methicillin-resistant Staphylococcus aureus), one of the most notorious hospital superbugs.
The combination of ineffective washing temperatures and bacterial adaptation creates a dangerous cycle: Healthcare workers’ uniforms can transport pathogens from hospitals to home washing machines, where inadequate cleaning allows these microbes to survive, contaminate the machine, spread to other clothes, and potentially return to healthcare environments.
This isn’t just a theoretical concern. Previous outbreaks have been linked to contaminated washing machines. In 2012, surgical scrubs contaminated by a healthcare worker’s home washing machine caused post-surgical infections in three patients. In 2019, an extended-spectrum beta-lactamase-producing Klebsiella oxytoca strain spread through a neonatal ward via knitted clothing washed in an on-site domestic machine.
Breaking the Cycle
Healthcare workers should avoid quick wash cycles, aim for temperatures above 70°C (158°F) to ensure reaching at least 60°C, clean their machines regularly, and replace them every four years. But the most effective solution might be moving away from home laundering altogether.
The researchers advise that professional commercial laundering services following proper standards would provide more reliable decontamination, improving the washing process and lowering infection transmission risks.
With antimicrobial resistance already causing over 1.2 million deaths annually worldwide, addressing every potential pathway for resistance development is crucial. This study suggests that something as seemingly innocent as washing work clothes at home might be contributing to one of healthcare’s most pressing crises.
Paper Summary
Methodology
Researchers evaluated the performance of six domestic laundry machines (DLMs) using Enterococcus faecium bioindicators to test decontamination efficacy. They monitored temperature during both full-length and rapid wash cycles at 60°C using biological and non-biological detergents. Additionally, they analyzed the microbiome and resistome of 12 DLMs through shotgun metagenomic sequencing. The team also conducted laboratory experiments exposing Staphylococcus aureus, Klebsiella pneumoniae, and Pseudomonas aeruginosa to sublethal concentrations of domestic detergents, increasing the concentration over time to induce tolerance. They then tested these detergent-tolerant bacteria for antibiotic resistance and performed whole genome sequencing to identify genetic mutations.
Results
Only 50% of DLMs achieved sufficient decontamination (≥5 log10 CFU reduction) during full-length cycles at 60°C, with rapid cycles performing even worse. Microbiome analysis revealed potentially pathogenic bacteria in the machines, including Mycobacterium, Pseudomonas, and Acinetobacter species, along with genes encoding for antibiotic resistance mechanisms. Bacteria exposed to detergents developed significant tolerance increases—up to 89,000% for S. aureus with liquid detergent and over 1.5 million percent for K. pneumoniae. More concerning, these detergent-tolerant bacteria developed cross-resistance to medically important antibiotics. K. pneumoniae became resistant to carbapenems, while S. aureus developed resistance patterns resembling MRSA. Whole genome sequencing identified mutations in genes encoding efflux pumps (MgrA in S. aureus and AcrB in K. pneumoniae) after detergent exposure.
Limitations
The study examined a relatively small sample of washing machines (six for performance testing and 12 for microbiome analysis). The machines tested were from standard households, not specifically from healthcare workers’ homes, which might have different microbial profiles. The laboratory conditions for detergent tolerance testing might not perfectly replicate real-world conditions in washing machines. Additionally, the study was conducted in the UK, where laundering practices and guidelines may differ from other countries.
Funding and Disclosures
The research was funded by De Montfort University and the Textile Services Association. The authors declared no competing interests.
Publication Information
The study “Domestic laundering of healthcare textiles: Disinfection efficacy and risks of antibiotic resistance transmission” was published in PLOS One on April 30, 2025, by Caroline Cayrou, Katie Silver, Lucy Owen, Jake Dunlop, and Katie Laird from the Infectious Disease Research Group at De Montfort University, Leicester, United Kingdom. The paper is available under open access terms.
So there is nothing short of getting rid of the washer that can stop these bacteria? That means one cannot sell the washer as it would infect the buyer’s clothing! Would running an empty hot cycle with bleach kill the bacteria? Chlorine kills everything. Some more research into disinfecting the home washer would be useful.